Detection of virulent thermophilic Campylobacter species in communal chickens

Marie E.A. Bissong; Collins N. Ateba

doi:10.17159/sajs.2019/4771

Authors

Marie E.A. Bissong 1. Department of Microbiology, School of Biological Sciences, North-West University, Mmabatho, South Africa; 2. Department of Biomedical Science, University of Bamenda, Bambili, Cameroon https://orcid.org/0000-0003-2132-7363
Collins N. Ateba 1. Department of Microbiology, School of Biological Sciences, North-West University, Mmabatho, South Africa; 2. Food Security and Safety Niche Area, Faculty of Agriculture, Science and Technology, North-West University, Mmabatho, South Africa https://orcid.org/0000-0003-1230-5138

DOI:

https://doi.org/10.17159/sajs.2019/4771

Keywords:

Campylobacter, virulence, genotype, prevalence, chicken

Abstract

Campylobacter is a foodborne pathogen found in the gut of poultry and other animals, which thereby act as reservoirs for human infection. Campylobacter produces various virulence factors which contribute to the establishment of disease. The aim of this study was to detect virulence genes in Campylobacter isolated from chicken faeces. A total of 408 poultry cloacal samples were analysed through culture, PCR and DNA sequencing. Campylobacter were detected in 24 (5.9 %) of the samples, of which 21 were C. jejuni while 3 were C. coli. The cdtB gene was detected in all Campylobacter isolates while cdtA and cdtC genes were detected in 20 (83.3%) and 18 (75.0%) of the isolates, respectively. The cadF gene was detected in 18 (85.7%) C. jejuni but not in C. coli. Eight genotypes designated G1 to G8 were detected among the isolates. Genotypes G1 and G7 possessed all three cdt genes, a potential for producing functionally active toxins. A large proportion (70.8%) of the isolates possessed both genes for toxin production and adhesion. The high prevalence of cdt and cadF genes in Campylobacter species in this study indicates the pathogenic potential of the isolates. There is a need to implement control measures to curb the spread of these virulent strains.

Significance:

Virulent thermophilic campylobacters cause severe health complications in humans.
Although chicken meat is the main source of human infections, there is limited data on the occurrence of Campylobacter in chicken faeces in southern Africa.
This study adds to literature on the occurrence of virulent strains of Campylobacter species, notably through the deposition of the DNA sequences of our local strains into GenBank®.
Communal chickens may serve as a source of virulent thermophilic campylobacters to humans, resulting in serious public health implications.

Open data sets:

MF872608 - https://www.ncbi.nlm.nih.gov/nuccore/MF872608.1
MF872609 - https://www.ncbi.nlm.nih.gov/nuccore/MF872609.1
MF872610 - https://www.ncbi.nlm.nih.gov/nuccore/MF872610.1
MF872611 - https://www.ncbi.nlm.nih.gov/nuccore/MF872611.1
MF872612 - https://www.ncbi.nlm.nih.gov/nuccore/MF872612.1