Evaluation of the antioxidant and antimicrobial activities of fucoxanthin from Dilophys fasciola and as a food additive in stirred yoghurt

Eman A. Ibrahim; Samah M. El-Sayed; Soha A. Murad; Walid E. Abdallah; Hoda S. El-Sayed

doi:10.17159/sajs.2023/13722

Authors

Eman A. Ibrahim Plant Biochemistry Department, National Research Centre, Cairo, Egypt https://orcid.org/0000-0002-3120-7667
Samah M. El-Sayed Dairy Department, National Research Centre, Cairo, Egypt https://orcid.org/0000-0003-3568-7546
Soha A. Murad Plant Biochemistry Department, National Research Centre, Cairo, Egypt https://orcid.org/0000-0001-7649-4391
Walid E. Abdallah Chemistry of Medicinal Plants Department, National Research Centre, Cairo, Egypt https://orcid.org/0000-0002-4183-0137
Hoda S. El-Sayed Dairy Department, National Research Centre, Cairo, Egypt https://orcid.org/0000-0003-1560-5523

DOI:

https://doi.org/10.17159/sajs.2023/13722

Keywords:

fucoxanthin, antioxidant, antimicrobial, probiotics, stirred yoghurt, physiochemical analysis, sensory evaluation

Abstract

We investigated the effects of fucoxanthin isolated from the edible macroalga Dilophys fasciola on pathogenic microbes and probiotics in vitro and the antioxidant activity of fucoxanthin. The yield concentration of the obtained crude was 50.5% fucoxanthin. We found strong inhibition against Gram-positive Staphylococcus aureus and Listeria monocytogenes, and lower inhibition against Gram-negative bacteria and fungi. The probiotic strains progressed between 1.2 and 1.67 log cycles at a concentration of 30 μg/mL. The antioxidant activity ranged between 54.76% and 88.36% at a concentration of 40 μg/mL. The 50% lethal dose of algal fucoxanthin was shown to be more than 2511.88 mg/kg. The production of stirred yoghurt incorporated with 20 mg and 30 mg of fucoxanthin per kilogram of milk was evaluated through chemical, microbiological, and sensory analyses during storage for 21 days and compared with control samples. The maximum growth for probiotics (Bifidobacterium bifidum and Lacticaseibacillus casei) was found on day 14, but more viability counts were detected in the treatment with 30 mg/kg. All treatments were free from mould and yeast counts up to 7 days, and the small numbers of mould, yeast, and psychrotrophic counts appeared first in control samples. Also, the highest dry matter content was observed for treatments with 30 mg/kg. Moreover, the protein, fat, and ash content of all treatments increased with a progressive cold storage period. Greater reductions in the pH were found in treatments than in the control, and were consistent with the development of acidity. During storage, the amount of crude fucoxanthin had no significant impact on the flavour, colour, or appearance scores.

Significance:

Fucoxanthin is a type of carotenoid that offers many benefits to human health.
The fucoxanthin of edible Dilophys fasciola had a strong antimicrobial effect against Gram-positive bacteria, Gram-negative bacteria, and fungi.
Stirred yoghurt fortified with crude fucoxanthin had good overall acceptability and the percentage of crude fucoxanthin had no noticeable effects on the flavour, colour, or appearance. Fucoxanthin, therefore, has potential benefit as a food additive.