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The family Equidae includes a diversity of extant species in the single genus Equus, notably horses (E. caballus and E. przewalskii) and a group
called the non-caballines (which includes both Asian and African asses, as well as African zebras). The fossil record, however, shows even more
remarkable diversity: within the past 5 million years at least 12 equid genera have been documented, including Hipparion, which is known from
Plio-Pleistocene deposits in the Cradle of Humankind World Heritage Site in South Africa. A linear evolutionary sequence from a hyracotherian
ancestor was proposed by Othaniel Marsh and championed by Thomas Huxley, but the evolution of equids within the past 55 million years is now
recognized as being much more complex. Palaeontologists such as Basil Cooke, Rufus Churcher, Vera Eisenmann and Alan Gentry have generally relied
on morphology and measurements for purposes of addressing the taxonomy of southern African Equidae, as others have done for equids from other areas.
More recently the potential of genetic data has been demonstrated, most remarkably in a study published in December by
Orlando et al.1
In the 1980s, Higuchi et al.2,3 succeeded in isolating mitochondrial DNA from the tissue of a museum specimen of the partially striped
South African Cape ‘quagga’, which became extinct in the 19th century. The genetic results stimulated a great deal of discussion as to
whether the Cape quagga was distinct at the species level from the more completely striped Burchell’s zebra.4,5
Genetic techniques to analyse modern and ancient DNA have improved dramatically within the past 25 years. A striking demonstration of this was
Orlando et al.’s successful recovery of a DNA from 22 of 35 teeth of Equus, dating to the Late Pleistocene or Holocene. Four of the teeth
which were successfully analysed for ancient DNA were attributed to E. capensis, a species which has been thought to have become extinct in
South Africa in the Late Quaternary. Other fossil equid specimens in the analysis were from sites in Europe, Asia and the American continents.
The late Reinhold Rau, taxidermist at the South African Museum (now part of the Iziko Museums of Cape Town), was delighted by the initial
mitochondrial DNA analyses because he had observed a gradation in the degree of striping from north to south, which suggested clinal variation
without a clear boundary between two taxa.6,7 Similar sentiments were expressed by Thackeray4,5 on the basis of variation in the
entoflexid of a lower premolar (P₄). He used modern museum specimens of equids from the Transvaal Museum (Northern Flagship Institution),
the Natural History Museum (London) and the American Museum of Natural History (New York) for comparative purposes, as well as fossil equid
specimens from Wonderwerk Cave, near Kuruman in the Northern Cape Province of South Africa, which has a long Quaternary sequence. Wonderwerk
is particularly interesting, as it is situated at the northern limit of the distribution of the partially striped ‘quagga’ and close
to the southern limit of that of the plains zebra. The latter was formerly classified as E. burchelli, but was reclassified as E. q. burchelli,
distinct at a subspecies level from the historically extinct E. q. quagga on the basis of mitochondrial DNA analyses by Higuchi et al.
2,3
The extraordinary ancient DNA results by Orlando et al.1 were published in a recent issue of the Proceedings of the National Academy of
Sciences. They were obtained from two separate laboratories: one in Lyon in France under Ludovic Orlando himself and the other, at Adelaide at the
Australian Centre for Ancient DNA, under the direction of Alan Cooper. The paper was co-authored by a team of 21 other researchers from many parts
of the world.
After checking and controlling for contamination, the team used the DNA results to explore phylogenetic relationships. The DNA clearly separated
Old World non-caballine equids from caballine and New World horses. In the case of Africa, the four specimens attributed to E. capensis (three
from Wonderwerk Cave, and one from Glen Craig Shelter near Port Elizabeth) grouped closely with specimens attributed to E. q burchelli and E. q
quagga. Specimens of the latter taxon were obtained from the Iziko Museums (South Africa), the Yale Peabody Museum (New Haven, USA), as well as
the Darmstadt and Berlin museums in Germany.
The authors noted that the results suggested ‘limited reproductive isolation (if any) amongst Cape quaggas and plains zebras’.
1 This view, based on a limited sample of ‘Cape quaggas’, is not in conflict with the opinion of a north-south clinal
variation expressed by Rau6,7 and Thackeray4,5. However, the genetic data do not support Churcher’s8
view that E. capensis and E. greyvi (from East Africa) were conspecific. Equus capensis is believed to have, instead, ‘formed part of the same
diverse taxonomic group as plains zebras and quaggas’, and ‘possessed a marked plasticity’ in terms of morphology and size.
1
Equus greyvi from Ethiopia and Kenya appears to group more closely with Asian asses than with the South African zebras. The mountain zebra
(E. hartmannae) does not group with E q. quagga or E. q. burchelli. Instead, the latter two taxa are more closely related to each other than
either is to the mountain zebra, E. hartmannae.
These exciting results constitute an important milestone in the growing understanding of equid phylogeny, not only in terms of African zebras,
but also in relation to Late Quaternary equids from Europe, Asia and the Americas. The study adds credibility to ancient DNA studies, given
appropriate sampling and preparation techniques.
Sites such as Wonderwerk Cave in South Africa have excellent preservation. This cave has yielded extinct ungulate horn samples with keratin
believed to be as old as 500,000 years BP, if not older.9 Keratin samples are currently being acquired for DNA analyses to supplement
existing results obtained from this site. Wonderwerk is believed to have a cave sequence extending back 2 million years. At Wonderwerk,
preservation is such that nitrogen, as well as carbon stable isotopes, can be measured in equid teeth.10 Orlando et al.1
have set the stage for exciting new developments in palaeogenetics, with special reference to South African fossils.(Back to the top)
1. Orlando L, Metcalf JL, Alberdi MT, et al. Revising the recent evolutionary history of equids using ancient DNA. Proc Natl Acad
Sci U S A.
2009;106:21754−21759.
2. Higuchi R, Bowman B, Freiberger M, Ryder OA, Wilson AC. DNA sequences from the quagga, an extinct member of the horse family. Nature. 1984;
312:282−284.3. Higuchi RG, Wrischnick LA, Oakes E, George M, Tong B, Wilson AC. Mitochondrial DNA of the extinct quagga: Relatedness and extent of postmortem
change. J Mol Evol. 1987;25:283−287. 4. Thackeray JF. Zebras from Wonderwerk Cave, northern Cape Province: Attempts to distinguish Equus burchelli and E. quagga. S Afr J Sci.
1988;84:99−101. 5. Thackeray JF. Morphometric, palaeoecological and taxonomic considerations of southern African zebras: Attempts to distinguish the quagga.
S Afr J Sci. 1997;93:89−93. 6. Rau RE. Revised list of the preserved material of the extinct Cape Colony Quagga, Equus quagga quagga (Gmelin). Ann S Afr Mus. 1974;65:41−
86. 7. Rau RE. Additions to the revised list of preserved material of the extinct Cape Colony Quagga and notes on the relationship and
distribution of southern Plains Zebras. Annals of the South African Museum, 1978;77:27−45. 8. Churcher CS. The extinct Cape zebra. Sagittarius. 1986;1:4−5. 9. Thackeray JF, Brink JS. Damaliscus niro horns from Wonderwerk Cave and other Pleistocene sites: Morphological and chronological considerations.
Palaeontol Afr. 2004;40:89−93. 10. Thackeray JF, Lee-Thorp JA. Isotopic analysis of equid teeth from Wonderwerk Cave, northern Cape Province, South Africa. Palaeogeogr
palaeoclimateol palaeoecol. 1992;99:141−150.
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